Related Experiment Video
Updated: Mar 15, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
Analyzing Synthetic Promoters Using Arabidopsis Protoplasts
Ralf Stracke1, Katharina Thiedig1, Melanie Kuhlmann1
1Genome Research, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
Abstract:
This chapter describes a transient protoplast co-transfection method that can be used to quantitatively study in vivo the activity and function of promoters and promoter elements (reporters), and their induction or repression by transcription factors (effectors), stresses, hormones, or metabolites. A detailed protocol for carrying out transient co-transfection assays with Arabidopsis At7 protoplasts and calculating the promoter activity is provided.
More Related Videos
10:10Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
10:58Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017