Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robotic subtotal gastrectomy with Hugo™ robotic-assisted surgery (RAS) system: first report on a case series.

International journal of surgery case reports·2026
Same author

Search for Leptonic Decays of Dark Photons at NA62.

Physical review letters·2024
Same author

Habitat suitability modelling to predict the distribution of deep coral ecosystems: The case of Linosa Island (southern Mediterranean Sea, Italy).

Marine environmental research·2024
Same author

Highly purified cannabidiol in the treatment of drug-resistant epilepsies: A real-life impact on seizure frequency, quality of life, behavior, and sleep patterns from a single Italian center.

Epilepsy & behavior : E&B·2023
Same author

Transient ischemic attack and minor stroke as "surgeons affairs": a narrative review.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2023
Same author

Data of postoperative complications related to fibrinogen-to-albumin ratio in pancreatic resections.

Data in brief·2022

Related Experiment Video

Updated: Mar 15, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

7.9K

Multifunctional System-on-Glass for Lab-on-Chip applications.

G Petrucci1, D Caputo1, N Lovecchio1

  • 1Department of Information Engineering, Electronics and Telecommunications, University of Rome "La Sapienza", via Eudossiana 18, Rome, 00184 Italy.

Biosensors & Bioelectronics
|August 28, 2016
PubMed
Summary

This study introduces a novel System on-Glass integrating thin film technologies for on-chip thermal treatments and biomolecule detection. This multifunctional platform enables efficient DNA amplification using Polymerase Chain Reaction (PCR) on a single site.

Keywords:
Amorphous silicon sensorsLab-on-ChipMicrofluidicsPCRThin film heater

More Related Videos

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
10:05

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Published on: April 28, 2015

29.8K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K

Related Experiment Videos

Last Updated: Mar 15, 2026

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods
07:51

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

7.9K
The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
10:05

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Published on: April 28, 2015

29.8K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K

Area of Science:

  • Microfluidics and Lab-on-Chip Technology
  • Thin Film Technology and Device Integration
  • Biomolecular Analysis and Diagnostics

Background:

  • Lab-on-Chip devices offer miniaturized biomolecular analysis with reduced time and reagent use.
  • Integrating multiple functions onto a single chip presents significant technological challenges.
  • Existing platforms often require complex external components for thermal control and detection.

Purpose of the Study:

  • To develop a multifunctional platform on a single glass substrate for integrated on-chip thermal treatments and biomolecule detection.
  • To demonstrate the feasibility of integrating diverse thin film technologies, including heaters, temperature sensors, and photosensors.
  • To enable on-chip DNA amplification via Polymerase Chain Reaction (PCR) with precise thermal cycling.

Main Methods:

  • Fabrication of a System on-Glass incorporating thin metal film heaters, hydrogenated amorphous silicon diodes (acting as temperature and photosensors), and a ground plane.
  • Optimization of technological steps, deposition temperatures, and photolithographic processes for seamless integration.
  • Coupling the System on-Glass with a microfluidic network (polydimethylsiloxane) containing microvalves and a PCR chamber.
  • Integration with an electronic system for driving heaters and controlling sensors.

Main Results:

  • Successful integration of multiple thin film technologies onto a single glass substrate.
  • Demonstration of precise on-chip thermal cycling for Polymerase Chain Reaction (PCR).
  • Verification of successful DNA amplification off-chip using a standard fluorometer.

Conclusions:

  • The developed System on-Glass is a multifunctional platform enabling on-chip thermal treatments and biomolecule detection.
  • The integration of thin film technologies overcomes previous limitations in Lab-on-Chip device functionality.
  • This platform shows significant potential for advancing miniaturized diagnostic and analytical systems, particularly for applications like PCR-based DNA amplification.