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Updated: Mar 15, 2026
Construction and Characterization of a Novel Vocal Fold Bioreactor
Published on: August 1, 2014
Quantification of change in vocal fold tissue stiffness relative to depth of artificial damage
Anna-Katharina Rohlfs1, Sebastian Schmolke1, Till Clauditz2
1a Department of Voice, Speech and Hearing Disorders , University Medical Center Hamburg-Eppendorf , Germany.
Objectives:
To quantify changes in the biomechanical properties of human excised vocal folds with defined artificial damage.
Methods:
The linear skin rheometer (LSR) was used to obtain a series of rheological measurements of shear modulus from the surface of 30 human cadaver vocal folds. The tissue samples were initially measured in a native condition and then following varying intensities of thermal damage. Histological examination of each vocal fold was used to determine the depth of artificial alteration. The measured changes in stiffness were correlated with the depth of cell damage.
Results:
For vocal folds in a pre-damage state the shear modulus values ranged from 537 Pa to 1,651 Pa (female) and from 583 Pa to 1,193 Pa (male). With increasing depth of damage from the intermediate layer of the lamina propria (LP), tissue stiffness increased consistently (compared with native values) following application of thermal damage to the vocal folds. The measurement showed an increase of tissue stiffness when the depth of tissue damage was extending from the intermediate LP layer downwards.
Conclusions:
Changes in the elastic characteristics of human vocal fold tissue following damage at defined depths were demonstrated in an in vitro experiment. In future, reproducible in vivo measurements of elastic vocal fold tissue alterations may enable phonosurgeons to infer the extent of subepithelial damage from changes in surface elasticity.
