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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment.

José A Jiménez-Torres1, David J Beebe1, Kyung E Sung2

  • 1Division of Cellular and Gene Therapies, Office of Cellular, Tissue, and Gene Therapies, Center for Biologics Evaluation and Research, The US Food and Drug Administration, Silver Spring, MD, USA.

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|September 2, 2016
PubMed
Summary

Researchers developed a new method using poly-dimethylsiloxane (PDMS) micro-molds to create controllable 3D luminal structures. This advance aids cancer research by mimicking in vivo tubular systems for studying cellular responses.

Keywords:
3D cell cultureLuminal structureMicrofluidicsOrganotypic modelTumor microenvironment

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cancer Research

Background:

  • Microscale 3D in vitro systems offer enhanced cellular microenvironment organization and throughput for cancer research.
  • Lumens, or tubular structures, are vital in vivo components of various tissues and are implicated in normal and disease processes like cancer development.
  • Existing methods lack practical approaches for creating diverse lumen structures needed to study cellular responses in cancer progression.

Purpose of the Study:

  • To present a novel, practical method for fabricating multiple three-dimensional (3D) luminal structures.
  • To enable precise control over lumen size, geometry, and spacing for advanced in vitro modeling.
  • To facilitate investigations into cellular behavior within controlled luminal microenvironments during cancer development.

Main Methods:

  • Utilized simple poly-dimethylsiloxane (PDMS) micro-molds for fabrication.
  • Engineered microscale 3D in vitro systems.
  • Controlled key parameters including lumen size, geometry, and inter-structure distance.

Main Results:

  • Successfully created multiple 3D luminal structures with tunable dimensions.
  • Demonstrated ease of control over structural parameters using PDMS micro-molds.
  • Established a versatile platform for mimicking in vivo lumen formation and architecture.

Conclusions:

  • The developed method provides a practical and controllable approach to generating 3D luminal structures.
  • This technique advances the capabilities of microscale 3D in vitro systems for cancer research.
  • Enables detailed study of cellular responses to microenvironmental cues within engineered lumens.