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Updated: Mar 15, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Investigation of non-linear imaging in high-resolution transmission electron microscopy
Yunjie Chang1, Yumei Wang1, Yanxiang Cui1
1Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Abstract:
Transmission cross-coefficient theory and pseudo-weak-phase object approximation theory were combined to investigate the non-linear imaging in high-resolution transmission electron microscopy (HRTEM). The analytical expressions of linear and non-linear imaging components in diffractogram were obtained and changes of linear and non-linear components over sample thickness were analyzed. Moreover, the linear and non-linear components are found to be an odd and even-function of the defocus and Cs, respectively. Based on this, a method for separating the linear and non-linear contrasts in Cs-corrected (non-zero Cs conditions included) HRTEM images was proposed, and its effectiveness was confirmed by image simulations with AlN as an example.
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