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Isolation of Epithelial Cells from Human Dental Follicle
Published on: November 5, 2021
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Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
Ritsuko Morita1, Miho Kihira2, Yousuke Nakatsu2
1Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan.
Plos One
|September 3, 2016
Summary
This study reveals how cell movement and division coordinate during tooth development. Actin remodeling, regulated by cofilin, is crucial for shaping tooth epithelium through cellular dynamics.
Area of Science:
- Developmental Biology
- Cell Biology
- Biophysics
Background:
- Ectodermal organ morphogenesis involves complex deformation modes like invagination and elongation.
- Coordination of multicellular dynamics during these processes is not fully understood.
Purpose of the Study:
- To investigate the coordination of cellular dynamics during tooth epithelial morphogenesis.
- To elucidate the role of cell movement, division, and actin remodeling in shaping ectodermal organs.
Main Methods:
- Developed a four-dimensional (4D) analysis system for single-cell resolution tracking of cell movement and division in developing tooth epithelium.
- Utilized a Fucci probe to analyze region- and stage-specific cell cycle patterns.
- Performed tissue-level deformation analysis to estimate volume growth rates.
Main Results:
- Cell cycle patterns correlated with volume growth rates in the tooth germ.
- Higher cellular motility was observed in regions with higher growth rates.
- Mitotic orientation was biased along the direction of tissue elongation.
- Spatio-temporal patterns of cellular dynamics and deformation correlated with cofilin activity.
Conclusions:
- Actin remodeling, influenced by cofilin, plays a significant role in coordinating cellular dynamics for tooth epithelial morphogenesis.
- The developed 4D system provides novel insights into cellular behavior coordination during ectodermal organogenesis, surpassing limitations of traditional histological analyses.
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