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Updated: Mar 15, 2026
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
23.4K
CRISPR-Cas: Revolutionising genome engineering.
Samantha Anne Nicholson1, Michael Sean Pepper
1Institute for Cellular and Molecular Medicine, South African Medical Research Council Extramural Unit for Stem Cell Research and Therapy, and Department of Immunology, Faculty of Health Sciences, University of Pretoria, South Africa. michael.pepper@up.ac.za.
South African Medical Journal = Suid-Afrikaanse Tydskrif Vir Geneeskunde
|September 8, 2016
Summary
Genome engineering technologies like CRISPR-Cas offer a future for repairing the human genome. This article explores CRISPR-Cas advancements, its medical potential, and ethical considerations.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Genome engineering, once science fiction, is becoming a clinical reality.
- Customizable sequence-specific endonuclease technologies are driving this progress.
Purpose of the Study:
- Discuss recent advancements in CRISPR-Cas technology.
- Explore the revolutionary potential of CRISPR-Cas in molecular medicine.
- Examine the ethical and regulatory challenges of CRISPR-Cas application.
Main Methods:
- Review of recent scientific literature on CRISPR-Cas technology.
- Analysis of CRISPR-Cas mechanisms and applications.
- Discussion of ethical frameworks and regulatory landscapes.
Main Results:
- CRISPR-Cas technology has rapidly advanced, offering unprecedented precision in genome editing.
- Significant potential exists for CRISPR-Cas to revolutionize treatments for genetic diseases.
- Substantial ethical and regulatory hurdles need to be addressed for clinical implementation.
Conclusions:
- CRISPR-Cas represents a transformative technology in molecular medicine.
- Careful consideration of ethical implications and robust regulatory frameworks are crucial for responsible application.
- Continued research and dialogue are essential to harness the full potential of genome engineering.
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