Serotyping, Genotyping, and Antimicrobial Susceptibility of Ornithobacterium rhinotracheale Isolates from Mexico

Area of Science:

• Veterinary Microbiology
• Poultry Disease Research
• Bacteriology

Background:

• Ornithobacterium rhinotracheale (ORT) is a significant pathogen causing respiratory disease and septicemia in poultry.
• ORT infections pose economic challenges to the poultry industry worldwide.
• Understanding the genetic and antimicrobial resistance profiles of ORT is crucial for effective disease management.

Purpose of the Study:

• To serotype and genotype Ornithobacterium rhinotracheale isolates from diseased poultry in Mexico.
• To determine the antimicrobial susceptibility patterns of these ORT isolates and reference strains.
• To provide data for developing control strategies against ORT infections in Mexican poultry.

Main Methods:

• Serotyping and genotyping of 9 reference strains and 23 ORT isolates from Mexican poultry.
• Antimicrobial susceptibility testing using standard microbiological methods.
• Comparative analysis of genotypic and phenotypic data.

Main Results:

• All 23 Mexican ORT isolates belonged to serotype A.
• A clonal relationship was observed among the studied ORT isolates.
• All tested ORT strains, including isolates and reference strains, exhibited resistance to colistin, fosfomycin, gentamicin, kanamycin, streptomycin, and trimethoprim-sulfamethoxazole.

Conclusions:

• The prevalence of serotype A and clonal nature of ORT in Mexico suggests a potential common source or successful dissemination of specific strains.
• The widespread antimicrobial resistance observed highlights the need for judicious antibiotic use in poultry.
• These findings are essential for informing targeted interventions and control programs for ORT in Mexican poultry populations.