Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.5K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.5K
Mismatch Repair01:20

Mismatch Repair

6.9K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.9K
Mismatch Repair01:36

Mismatch Repair

44.5K
Overview
44.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Different Polysorbates (Tween) on Recombined Whipped Cream: Interfacial Properties, Stability, and Aeration Properties.

Foods (Basel, Switzerland)·2026
Same author

Early menopause and cardiovascular disease risk: triangulating the evidence with a combined analysis.

Climacteric : the journal of the International Menopause Society·2026
Same author

Tyramine-mediated aggregation amplification of Au@Ag nanocubes Raman tags for sensitive multiplex SERS biosensing.

Talanta·2026
Same author

Does Depression Increase the Risk of Endometriosis? A Combined Population‑Based and Laboratory Study.

International journal of women's health·2026
Same author

The obesity paradox effect of triglyceride-glucose-body mass index (TyG-BMI) on all-cause mortality in patients with malignant tumors: a machine learning-driven cohort study.

Translational cancer research·2026
Same author

Engineered haptoglobin β fusion protein targets myoglobin and ameliorates rhabdomyolysis-associated acute kidney injury.

EMBO molecular medicine·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Mar 15, 2026

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

8.2K

Probing RNA recognition by human ADAR2 using a high-throughput mutagenesis method.

Yuru Wang1, Peter A Beal2

  • 1Department of Chemistry, University of California, One Shields Ave, Davis, CA 95616, USA.

Nucleic Acids Research
|September 11, 2016
PubMed
Summary
This summary is machine-generated.

Adenosine deaminases acting on RNA 2 (ADAR2) is crucial for RNA modification. This study identified key residues in the ADAR2 5' binding loop essential for RNA recognition and enzyme function.

More Related Videos

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

8.5K
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

11.2K

Related Experiment Videos

Last Updated: Mar 15, 2026

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

8.2K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

8.5K
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

11.2K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Adenosine deamination is a prevalent mRNA post-transcriptional modification catalyzed by ADAR1 and ADAR2 in humans.
  • ADAR protein malfunction is linked to various diseases.
  • Previous work revealed a 5' binding loop in ADAR2 crucial for RNA interaction and substrate specificity.

Purpose of the Study:

  • To elucidate structure-activity relationships within the ADAR2 5' binding loop.
  • To deepen the understanding of RNA recognition mechanisms by ADAR2.
  • To identify specific amino acid residues critical for ADAR2 function.

Main Methods:

  • Development of a high-throughput mutagenesis approach for ADAR variants.
  • Screening of ADAR variants in single yeast cells.
  • Quantitative evaluation of enzymatic activity for structure-function analysis.

Main Results:

  • Detailed structure-activity relationships were established for the ADAR2 5' binding loop.
  • The importance of specific amino acids at 19 positions was determined.
  • Six essential residues were identified, contributing to loop structure and RNA binding.

Conclusions:

  • New insights into RNA recognition by ADAR2 were gained.
  • A novel tool was established for defining structure-function relationships in ADAR reactions.
  • This research advances the understanding of ADAR-mediated RNA editing.