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Respiratory Burst Process in Diabetic Children

Anahita Sanaei Dashti1, Soodabeh Taheri2, Reza Jouybar3

  • 1Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran.

Iranian Journal of Pediatrics
|September 13, 2016
PubMed

Insights

Diabetic children show a reduced respiratory burst in polymorphonuclear cells (PMNs), a key part of immune function. This impaired phagocytosis may explain their higher infection rates.

Area of Science:

  • Immunology
  • Pediatric Endocrinology

Background:

  • Diabetes mellitus (DM) is associated with an increased risk of infections.
  • Polymorphonuclear (PMN) functions are critical components of the immune system potentially affected in DM.

Purpose of the Study:

  • To investigate the respiratory burst process in PMNs of children with DM.
  • To assess the role of PMN phagocytosis in the pathophysiology of infections in diabetic children.

Main Methods:

  • A cross-sectional study involving 52 children with insulin-dependent diabetes and 29 non-diabetic controls.
  • Nitroblue tetrazolium (NBT) test performed on PMNs from heparinized blood samples.
  • Statistical analysis using SPSS version 16 with significance set at P < 0.05.

Main Results:

  • Mean NBT test results were significantly lower in diabetic children (72.1 ± 15.84) compared to non-diabetic children (94.68 ± 5.31) (P < 0.001).
  • No significant correlation was found between NBT levels and age, gender, diabetes duration, insulin dosage, or HbA1C levels.

Conclusions:

  • The respiratory burst function of PMNs is demonstrably impaired in children with diabetes.
  • This PMN dysfunction offers a potential mechanistic explanation for the heightened susceptibility to infections observed in pediatric DM.
Abstract

Keywords:
Diabetes MellitusNeutrophilsNitrobluetetrazoliumRespiratory Burst

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