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Related Experiment Video

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A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Profiling Kinase Activity during Hepatitis C Virus Replication Using a Wortmannin Probe.

Geneviève F Desrochers¹, Allison R Sherratt¹, David R Blais¹

¹Life Sciences Division, National Research Council of Canada , 100 Sussex Drive, Ottawa, Canada.

ACS Infectious Diseases

|September 13, 2016

Summary

This summary is machine-generated.

Hepatitis C virus (HCV) alters host cell kinases, crucial for its life cycle. Activity-based protein profiling revealed changes in kinase activity affecting viral entry, replication, and secretion.

Keywords:

MAPK pathway activity-based protein profiling hepatitis C host−virus interactions kinase probes wortmannin

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Last Updated: Mar 15, 2026

A Protocol for Analyzing Hepatitis C Virus Replication

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Published on: June 26, 2014

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

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A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

Area of Science:

Biochemistry
Virology
Cell Biology

Background:

Hepatitis C virus (HCV) manipulates host cell processes for its life cycle.
Kinases regulate critical pathways exploited by HCV, making them potential therapeutic targets.

Purpose of the Study:

To identify kinases with altered activity in HCV-infected cells using activity-based protein profiling (ABPP).
To understand how HCV modulates the host cell kinome to facilitate viral replication and propagation.

Main Methods:

Utilized wortmannin-yne, a probe based on the kinase inhibitor wortmannin, for ABPP.
Applied bioorthogonal chemistry for bioconjugation of the probe.
Quantified kinase activity in HCV-expressing human hepatoma cells.

Main Results:

Identified differential kinase activity in HCV-expressing cells.
Observed changes in kinases involved in MAPK, apoptosis, and cell cycle pathways.
Demonstrated modulation of the active kinome impacting viral entry, replication, assembly, and secretion.

Conclusions:

HCV actively remodels the host cell kinome to support its life cycle.
Targeting these altered kinase activities presents a potential strategy for HCV therapeutics.