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Poly r(C) binding protein is post-transcriptionally repressed by MiR-490-3p to potentiate squamous cell carcinoma

Shan Xia1,2, Zigang Zhao1, Fang Xie1

  • 1Department of Dermatology, Chinese PLA General Hospital, No. 28, Fuxing Road, Beijing, 100853, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|September 16, 2016
PubMed

Insights

Researchers discovered that microRNA-490-3p silences the expression of Poly r(C) binding protein 1 (PCBP1) in squamous cell skin cancer. Modulating miR-490-3p offers a potential therapeutic strategy for this deadly cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Squamous cell skin carcinoma is a significant cause of cancer mortality.
  • Poly r(C) binding protein 1 (PCBP1) acts as a tumor suppressor.
  • PCBP1 expression is inversely correlated with cancer stem cell maintenance in certain cancers.

Purpose of the Study:

  • To elucidate the regulatory mechanism of PCBP1 expression in squamous cell skin carcinoma.
  • To investigate the role of microRNAs in PCBP1 regulation.

Main Methods:

  • Analysis of PCBP1 protein expression in CD34+ COLO-16 cells.
  • Identification of microRNA targeting PCBP1 mRNA.
  • Exogenous manipulation of miR-490-3p levels.

Main Results:

  • Loss of PCBP1 protein expression in CD34+ COLO-16 cells is due to translational silencing.
  • PCBP1 mRNA is targeted by miR-490-3p, causing translational silencing.
  • Modulating miR-490-3p levels affects PCBP1 protein expression.

Conclusions:

  • miR-490-3p orchestrates the translational silencing of PCBP1 in squamous cell skin carcinoma.
  • miR-490-3p represents a potential biomarker and therapeutic target for squamous cell skin cancer.
Keywords:
MiR-490-3pPoly r(C) binding proteinPotentiate squamous cell carcinoma

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