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Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...

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Updated: Mar 15, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

A Three-Dimensional Origami Paper-Based Device for Potentiometric Biosensing.

Jiawang Ding¹, Bowei Li¹, Lingxin Chen¹

¹Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong, 264003, P.R. China.

Angewandte Chemie (International Ed. in English)

|September 17, 2016

Summary

This summary is machine-generated.

This study introduces a novel 3D origami paper device for simple, portable, and cost-effective potentiometric biosensing of proteins and small molecules, enabling in-situ measurements.

Keywords:

biosensors enzyme-based assays origami paper-based devices potentiometry

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Published on: November 22, 2016

Area of Science:

Analytical Chemistry
Biosensors
Materials Science

Background:

Paper-based potentiometric ion-sensing platforms are limited to planar designs for simple ion detection.
Existing platforms are not suitable for the complex biosensing of proteins or small molecules.
There is a need for portable, cost-effective biosensing solutions.

Purpose of the Study:

To develop a novel three-dimensional (3D) origami paper-based device for potentiometric biosensing.
To integrate solid-contact ion-selective and all-solid-state reference electrodes onto a paper platform.
To demonstrate the device's utility for detecting enzyme activities and small molecule inhibitors.

Main Methods:

Fabrication of a 3D origami device by impregnating paper with bioreceptors and reporting reagents.
Integration of solid-contact ion-selective electrode and all-solid-state reference electrode.
Utilizing a USB-controlled miniaturized electrochemical detector for in-situ measurements.
Application of the device for detecting butyrylcholinesterase activity and organophosphate pesticides.

Main Results:

Successful development of the first 3D origami paper-based potentiometric biosensing device.
Demonstrated versatility for potentiometric bioassays through folding and unfolding paper structures.
Achieved successful detection of enzyme activities and organophosphate pesticides using butyrylcholinesterase as a model.
Enabled simple, portable, and cost-effective in-situ measurements.

Conclusions:

The 3D origami paper device offers a novel platform for potentiometric biosensing.
The device facilitates the detection of proteins and small molecules in a portable and cost-effective manner.
This technology holds promise for various applications requiring simple and accessible biosensing.