Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging the hallmarks of cancer.

Nature reviews. Cancer·2026
Same author

Laparoscopic transabdominal retromuscular repair with hernia sac-assisted mesh coverage for incisional ventral hernia: a prospective case series with preliminary outcomes.

Journal of minimally invasive surgery·2026
Same author

Ethionamide versus ethambutol-containing first-line regimens for TB meningitis.

Antimicrobial agents and chemotherapy·2026
Same author

BCG vaccination mitigates tau pathology and restores cognitive function in PS19 mice.

bioRxiv : the preprint server for biology·2026
Same author

Next-Generation Implant Technologies: Breakthroughs, Barriers, and the Road Ahead.

Current pharmaceutical design·2026
Same author

Innovative Nanoparticle-based Therapeutic Strategies: Overcoming Biological Barriers for Enhanced Efficacy.

Current drug targets·2026

Related Experiment Video

Updated: Mar 15, 2026

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

8.8K

A Systematic Approach for Developing Bacteria-Specific Imaging Tracers.

Alvaro A Ordonez1,2, Edward A Weinstein1,3, Lauren E Bambarger1,2

  • 1Center for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
|September 17, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed novel imaging tracers targeting bacterial metabolism to detect infections, including multidrug-resistant organisms (MDROs). These pathogen-specific agents show promise for rapid clinical diagnosis and treatment monitoring.

Keywords:
PETbacteriadrug-resistanceimagingtranslational

More Related Videos

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues
08:01

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

1.5K
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

7.9K

Related Experiment Videos

Last Updated: Mar 15, 2026

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

8.8K
Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues
08:01

Author Spotlight: Unraveling Bacterial Responses to Antibiotics and Immune System in Tissues

Published on: March 1, 2024

1.5K
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Published on: February 26, 2018

7.9K

Area of Science:

  • Biochemistry
  • Medical Imaging
  • Infectious Diseases

Background:

  • Modern patients face increasing risks from bacterial infections, particularly multidrug-resistant organisms (MDROs).
  • Current diagnostic methods lack noninvasive, whole-body imaging for rapid infection source identification and treatment response monitoring.
  • There is a critical need for pathogen-specific imaging technologies in clinical settings.

Purpose of the Study:

  • To develop novel, noninvasive imaging tracers for pathogen-specific detection of bacterial infections.
  • To identify molecules that selectively target bacterial metabolic pathways for imaging purposes.
  • To evaluate the potential of these tracers for clinical translation in diagnosing infections caused by various bacteria, including MDROs.

Main Methods:

  • Systematic in silico screening of 961 radiolabeled molecules against essential bacterial metabolic pathways.
  • In vitro uptake studies using representative bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, mycobacteria).
  • Evaluation of fluorine-labeled analogs in a murine myositis model for PET imaging potential.

Main Results:

  • Identified three novel, nontoxic molecules (PABA, mannitol, sorbitol) with selective bacterial uptake and no mammalian cell accumulation.
  • Demonstrated rapid accumulation of these molecules in clinical isolates of MDROs, including MRSA and resistant Enterobacteriaceae.
  • Fluorine-labeled analogs successfully detected and differentiated infection sites from sterile inflammation in a murine model (P = 0.03).
  • 2-deoxy-2-[F-18]fluoro-d-sorbitol (18F-FDS) showed potential for PET imaging, differentiating infection from inflammation in mice.

Conclusions:

  • Developed a systematic approach to create pathogen-specific imaging tracers by targeting unique bacterial biochemical pathways.
  • These novel tracers have significant potential for clinical application in detecting and localizing a wide spectrum of bacterial infections, including MDROs.
  • The findings pave the way for improved diagnostic tools in infectious disease management.