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Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

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Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.7K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

6.6K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
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Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.7K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.7K
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

3.8K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
3.8K
¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

1.5K
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
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Related Experiment Video

Updated: Mar 14, 2026

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
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Physical characterization using diffusion NMR spectroscopy.

Mikhail Zubkov1, Gary R Dennis1, Tim Stait-Gardner1

  • 1Nanoscale Organisation and Dynamics Group, School of Science and Health, Western Sydney University, Penrith, NSW, Australia.

Magnetic Resonance in Chemistry : MRC
|September 23, 2016
PubMed
Summary
This summary is machine-generated.

Nuclear Magnetic Resonance diffusion measurements (dNMR) reveal solution organization and microgeometry by tracking molecular motion. Recent advances enhance dNMR

Keywords:
anisotropic diffusiondiffusion NMRhydrogen bondingisomer diffusionpolymer dynamicsrestricted diffusionsolution structuring

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Area of Science:

  • Physical Chemistry
  • Materials Science
  • Biophysics

Background:

  • Nuclear Magnetic Resonance (NMR) diffusion measurements (dNMR) are versatile tools for analyzing molecular motion.
  • Understanding solution organization and microgeometry is crucial across various scientific disciplines.

Purpose of the Study:

  • To introduce dNMR and summarize recent advancements in the field.
  • To showcase the broad applicability of dNMR across diverse systems and research topics.

Main Methods:

  • Probing random molecular motion using NMR diffusion measurements.
  • Applying various dNMR techniques to analyze restricted and anisotropic diffusion.
  • Developing advanced dNMR methods for enhanced sensitivity and speed.

Main Results:

  • Demonstrated dNMR's utility in studying polymer dynamics, solution structuring, and cell models.
  • Showcased applications in systems including liquid crystals, ionic liquids, and biological samples.
  • Highlighted the capability of dNMR to extract structural information from diffusing particles.

Conclusions:

  • dNMR is a powerful and adaptable technique for characterizing complex systems at the molecular level.
  • Recent developments expand dNMR's potential for probing larger geometries, dilute solutions, and rapid processes.