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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Updated: Mar 14, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Chromatin Immunoprecipitation.

Laura Wiehle¹, Achim Breiling²

¹Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany. l.wiehle@dkfz.de.

Methods in Molecular Biology (Clifton, N.J.)

|September 24, 2016

Summary

This summary is machine-generated.

Chromatin immunoprecipitation (ChIP) maps protein-DNA interactions in vivo. This method identifies binding sites for proteins like transcription factors and epigenetic modifiers across the genome.

Keywords:

ChIP Chromatin Cross-linking DNA-protein interactions Immunoprecipitation Repressive protein complexes

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Last Updated: Mar 14, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

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Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

Area of Science:

Molecular Biology
Epigenetics
Genomics

Background:

Chromatin immunoprecipitation (ChIP) is a fundamental technique for studying protein-DNA interactions within living organisms.
It has been crucial for identifying binding sites and patterns of various DNA-interacting proteins, including transcription factors, modified histones, and epigenetic modifiers.
The Polycomb repressors were initially mapped using ChIP, elucidating their biological functions.

Purpose of the Study:

To provide a detailed protocol for the standard Chromatin immunoprecipitation (ChIP) assay.
To discuss variations and applications of the ChIP technique.
To highlight the importance of ChIP in understanding protein-DNA interactions and epigenetic regulation.

Main Methods:

Chromatin immunoprecipitation (ChIP) involves fragmenting cross-linked (XChIP) or native (NChIP) chromatin from cells or tissues.
The protein of interest is immunoprecipitated using a specific antibody.
Co-precipitated DNA is purified and analyzed via PCR, ChIP-on-chip, or ChIP-sequencing (ChIP-seq).

Main Results:

ChIP assays can determine the localization of target proteins at specific DNA loci or across the entire genome.
The technique provides insights into the binding patterns of transcription factors, histone modifications, and epigenetic regulators.
Analysis can range from specific gene regions to whole-genome profiling.

Conclusions:

Chromatin immunoprecipitation (ChIP) is an indispensable tool for mapping protein-DNA interactions in vivo.
The method allows for detailed investigation of epigenetic mechanisms and gene regulation.
Standardized protocols and variations enable diverse applications from candidate gene analysis to genome-wide profiling.