Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Contaminants and Errors01:16

Contaminants and Errors

464
Effective sample preparation is crucial for accurate and reliable laboratory analysis. During this process, two significant sources of error can arise: concentration bias from improper sample splitting and contamination caused by methods used to reduce particle size, such as grinding or homogenization. Identifying and minimizing these potential errors is crucial to ensuring the validity of the analysis.
Another key consideration is determining the appropriate number of samples required to...
464
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

1.6K
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
1.6K
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

2.3K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
2.3K
Systematic Error: Methodological and Sampling Errors01:15

Systematic Error: Methodological and Sampling Errors

11.3K
In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
11.3K
Atomic Emission Spectroscopy: Interference01:30

Atomic Emission Spectroscopy: Interference

725
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
725
Blank Solutions00:56

Blank Solutions

1.4K
A blank solution is a solution that does not contain the analyte, or the substance of interest being tested or measured. It is typically prepared using the same reagents and procedure as the sample solution but without adding the analyte. The primary purpose of preparing a blank solution is to account for any background interference or contamination that may affect the accuracy and reliability of the analytical method.
In some experimental cases, the reagents, solvents, or lab equipment used in...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of interventions to improve test appropriateness.

Clinical chemistry and laboratory medicine·2022
Same author

The Relationship Between Intact Parathyroid Hormone and 25-Hydroxyvitamin D in United Kingdom Resident South Asians and Whites: A Comparative, Cross-Sectional Observational Study.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2021
Same author

Clinicians' and laboratory medicine specialists' views on laboratory demand management: a survey in nine European countries.

Diagnosis (Berlin, Germany)·2020
Same author

[Joint EFLM-COLABIOCLI recommendation for venous blood sampling].

Annales de biologie clinique·2019
Same author

Sample transportation - an overview.

Diagnosis (Berlin, Germany)·2019
Same author

Joint EFLM-COLABIOCLI Recommendation for venous blood sampling

Clinical chemistry and laboratory medicine·2018
Same journal

Evaluation of hemoglobin interference thresholds for chemical urinalysis panels.

Clinical biochemistry·2026
Same journal

Integrated genomic and biochemical diagnosis of a novel homozygous start-loss variant in AKR1D1 associated with neonatal cholestasis.

Clinical biochemistry·2026
Same journal

Agreement between POC glucose meters and blood gas analyzers varies across ICU patient populations.

Clinical biochemistry·2026
Same journal

From variability to value: Advancing vancomycin therapeutic drug monitoring toward decision-grade practice through commutability-informed external quality assessment and uncertainty-aware reporting.

Clinical biochemistry·2026
Same journal

Response to the letter to the editor: From variability to value: Advancing vancomycin therapeutic drug monitoring toward decision-grade practice through commutability-informed external quality assessment and uncertainty-aware reporting.

Clinical biochemistry·2026
Same journal

What are the correlates of laboratory productivity in clinical laboratories in the Asia Pacific region?

Clinical biochemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
09:09

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

10.3K

Exogenous sample contamination. Sources and interference.

Michael P Cornes1

  • 1Clinical Chemistry Department, Royal Wolverhampton NHS Trust, Wolverhampton WV10 0QP, UK.

Clinical Biochemistry
|September 25, 2016
PubMed
Summary
This summary is machine-generated.

Clinical laboratory medicine relies on accurate results, but external substances can cause interference. This review summarizes exogenous interferences and strategies to minimize their impact on patient care.

Keywords:
ExogenousInterferencePre-analytical

More Related Videos

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.6K
Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
08:26

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

2.1K

Related Experiment Videos

Last Updated: Mar 14, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
09:09

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

10.3K
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.6K
Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
08:26

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

2.1K

Area of Science:

  • Clinical laboratory medicine
  • Analytical chemistry
  • Pathology

Background:

  • Clinical laboratory medicine is integral to patient care, influencing 60-70% of critical decisions.
  • Accurate and precise laboratory results are essential for reflecting the in vivo patient status.
  • Assay complexity can lead to interference, affecting analytical results.

Purpose of the Study:

  • To define and categorize exogenous interferences in laboratory analysis.
  • To identify sources of exogenous substances that can impact laboratory results.
  • To outline strategies for minimizing the effects of exogenous interference.

Main Methods:

  • Review of existing literature on laboratory interferences.
  • Classification of interferences into endogenous and exogenous categories.
  • Analysis of how exogenous substances enter the sample pathway.

Main Results:

  • Exogenous interferences originate from outside the body and can introduce bias in analytical results.
  • These substances can contaminate specimens at various points in the sample journey.
  • Failure to detect interferences can negatively impact patient care, safety, and hospital finances.

Conclusions:

  • Laboratories must implement processes to identify and mitigate contamination and interference.
  • Collaboration between laboratories, clinicians, and manufacturers is crucial for managing interferences.
  • Proactive identification and management of exogenous interferences are vital for maintaining result quality.