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Updated: Mar 14, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Gene expression changes in bioceramic paste-treated human dental pulp cells.

Deniz Torun1, Zeynep Ö Torun, Kadriye Demirkaya

  • 1Department of Medical Genetics, Gulhane Military Medical Academy.

Journal of Oral Science
|September 27, 2016
PubMed
Summary

iRoot BP alters gene expression in human dental pulp cells, affecting metabolic, developmental, and regulatory processes. This study used microarray and PCR to analyze gene changes after 24 and 72 hours.

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Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Dental Research

Background:

  • Dental pulp cells are crucial for maintaining tooth vitality.
  • Understanding the biological effects of endodontic materials is essential for clinical success.
  • iRoot BP is a bioceramic root canal filling material.

Purpose of the Study:

  • To investigate the impact of iRoot BP on the gene expression profile of human dental pulp cells.
  • To identify specific biological pathways affected by iRoot BP exposure.

Main Methods:

  • Human dental pulp cells were exposed to iRoot BP.
  • Gene expression profiling was performed using microarray analysis at 24 and 72 hours.
  • Quantitative reverse transcriptase PCR (qRT-PCR) was used for verification.

Main Results:

  • Microarray analysis revealed significant changes in gene expression.
  • 21 transcripts were up-regulated and 15 were down-regulated by more than two-fold.
  • Upregulated genes were primarily involved in metabolic, developmental, and regulatory processes.
  • Downregulated genes were associated with cell communication, development, and biological regulation.

Conclusions:

  • iRoot BP significantly influences the gene expression of human dental pulp cells.
  • The material affects key biological processes, including metabolism, development, and cell communication.
  • These findings provide insights into the molecular interactions between iRoot BP and dental pulp tissue.