Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman...

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Spectroscopy

Background:

• Accurate glucose monitoring is crucial for diabetes management.
• Existing methods for glucose sensing face challenges in selectivity and real-time in vivo application.
• Development of novel biosensors with high sensitivity and specificity is needed.

Purpose of the Study:

• To demonstrate direct glucose sensing using surface-enhanced Raman spectroscopy (SERS) on functionalized gold film-over-nanosphere (AuFON) substrates.
• To evaluate the selectivity and accuracy of bisboronic acid receptors for glucose detection in complex biological samples.
• To explore the potential of SERS-based sensors for in vivo glucose monitoring.

Main Methods:

• Utilized gold film-over-nanosphere (AuFON) substrates functionalized with bisboronic acid receptors.
• Employed surface-enhanced Raman spectroscopy (SERS) for direct glucose detection.
• Applied multivariate statistical analysis to resolve glucose from interfering substances like fructose.
• Performed computational modeling to support vibrational frequency assignments.

Main Results:

• Achieved direct sensing of glucose at physiologically relevant concentrations (1-10 mM).
• Demonstrated high selectivity, enabling glucose resolution in the presence of high fructose backgrounds.
• Confirmed accurate glucose detection through combined SERS and multivariate statistical analysis.
• Computational modeling provided insights into molecular interactions and vibrational modes.

Conclusions:

• Bisboronic acid-functionalized AuFON substrates show significant promise for SERS-based glucose sensing.
• The developed sensor system offers high selectivity and accuracy for glucose detection.
• These findings support the potential application of this technology in non-invasive or minimally invasive in vivo glucose monitoring for diabetes management.