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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
Published on: October 25, 2018
Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA
Wen Wan1, Dongmei Wang1, Xiaolian Gao1,2
1School of Life Science, University of Science and Technology of China, No 443 Huangshan Road, Hefei, Anhui, 230026, People's Republic of China.
Abstract:
Applications of microchip-synthesized oligonucleotides for de novo gene synthesis are limited primarily by their high error rates. The mismatch binding protein MutS, which can specifically recognize and bind to mismatches, is one of the cheapest tools for error correction of synthetic DNA. Here, we describe a protocol for removing errors in microchip-synthesized oligonucleotides and for the assembly of DNA segments using these oligonucleotides. This protocol can also be used in traditional de novo gene DNA synthesis.
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