Related Experiment Video
Updated: Jul 16, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Analysis of warfarin and its metabolites by reversed-phase ion-pair liquid chromatography with fluorescence detection
1Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin 78712.
Abstract:
A high-performance liquid chromatographic method was developed for the determination of warfarin and its metabolites (diastereomeric warfarin alcohols and 6-, 7-, 8-, 4'- and 3'-hydroxywarfarin) in microbial cultures. Ion-pair chromatography with tetrabutylammonium ion as the counter ion allowed for the complete resolution of all compounds at pH 7.5 on a reversed-phase (C18) column, thus permitting direct fluorescence detection without the use of post-column pH switching techniques. Analysis of cell suspension cultures of the fungus Cunninghamella elegans (ATCC 36112) indicated that this organism metabolizes warfarin to all known mammalian metabolites, plus the previously unreported 3'-hydroxywarfarin. Detection limits for all compounds were in the low nanogram range.
More Related Videos
08:06Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Published on: October 25, 2019
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Ion-Exchange Chromatography
Therapeutic Drug Monitoring: Drug Analysis Methods