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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Immunoprecipitation01:20

Immunoprecipitation

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
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Western Blotting01:15

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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Related Experiment Video

Updated: Mar 14, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Antibody-Based Proteomics.

Christer Wingren1

  • 1Department of Immunotechnology, Lund University, Medicon Village, SE-223 81, Lund, Sweden. christer.wingren@immun.lth.se.

Advances in Experimental Medicine and Biology
|October 1, 2016
PubMed
Summary

Antibody-based proteomic approaches enable rapid, sensitive protein profiling for disease diagnostics and biomarker discovery. These methods create detailed molecular fingerprints for personalized medicine applications.

Keywords:
Affinity proteomicsAntibodyAntibody arraysAntibody-based proteomicsBiomarkerDisease proteomicsProtein expression profiling

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Area of Science:

  • Proteomics
  • Biotechnology
  • Molecular Biology

Background:

  • Antibody-based proteomic approaches are crucial for high-throughput, multiplexed protein expression profiling in health and disease.
  • These technologies offer specific, sensitive, and rapid simultaneous profiling of numerous proteins, including low-abundant ones in complex samples like serum.

Purpose of the Study:

  • To review the current status of antibody-based proteomic approaches, with a focus on antibody arrays.
  • To discuss the benefits, limitations, and applicative potential of these proteomic strategies.

Main Methods:

  • Focus on antibody arrays for multiplexed protein expression profiling.
  • Utilizing bioinformatics to analyze proteomic snapshots and identify biomarker signatures.

Main Results:

  • Protein expression patterns (proteomic snapshots) can be transformed into detailed molecular fingerprints (proteomic maps).
  • Bioinformatics enables deciphering of candidate biomarker signatures for clinical evaluation.

Conclusions:

  • Antibody-based proteomic approaches offer unique opportunities for disease diagnostics, biomarker discovery, patient stratification, and therapy selection.
  • These methods provide detailed molecular insights into proteomes, advancing personalized medicine.