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miR-1207-3p regulates the androgen receptor in prostate cancer via FNDC1/fibronectin

Dibash K Das1, Michelle Naidoo2, Adeodat Ilboudo2

  • 1Department of Biological Sciences, Hunter College of The City University of New York, New York, NY 10065, USA; The Graduate Center Departments of Biology and Biochemistry, The City University of New York, New York, NY 10016, USA; Department of Medicine, Weill Cornell Medicine, Cornell University, New York, NY 10065, USA.

Experimental Cell Research
|October 4, 2016
PubMed

Insights

MicroRNA-1207-3p is underexpressed in prostate cancer (PCa), inhibiting tumor growth and spread. Its pathway targets FNDC1, FN1, and AR, offering new therapeutic strategies for aggressive PCa.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer (PCa) is a prevalent malignancy in men.
  • MicroRNA (miRNA) dysregulation is common in various cancers.
  • MicroRNA-1207-3p, located at the PCa susceptibility locus 8q24, has an unclear role in PCa.

Purpose of the Study:

  • To investigate the role of microRNA-1207-3p in prostate cancer.
  • To identify the molecular targets and regulatory pathways of microRNA-1207-3p in PCa.

Main Methods:

  • Comparative analysis of microRNA-1207-3p expression in PCa cell lines versus normal prostate cells.
  • Functional assays to assess the impact of microRNA-1207-3p on PCa cell proliferation, migration, and apoptosis.
  • Molecular targeting analysis of FNDC1 by microRNA-1207-3p.
  • Expression analysis of FNDC1, fibronectin (FN1), and androgen receptor (AR) in PCa cell lines and patient tissues.

Main Results:

  • MicroRNA-1207-3p is significantly underexpressed in PCa cell lines.
  • Increased microRNA-1207-3p expression inhibits PCa cell proliferation and migration, and induces apoptosis by targeting FNDC1.
  • FNDC1, FN1, and AR are overexpressed in PCa and correlate with aggressive disease.
  • Elevated FN1 expression in tumors is associated with PCa-specific mortality.
  • FNDC1, FN1, and AR are co-overexpressed in metastatic PCa.

Conclusions:

  • A novel regulatory pathway involving microRNA-1207-3p, FNDC1, FN1, and AR in prostate cancer has been identified.
  • MicroRNA-1207-3p acts as a tumor suppressor in PCa by targeting FNDC1.
  • The microRNA-1207-3p/FNDC1/FN1/AR pathway represents a potential therapeutic target for aggressive and metastatic prostate cancer.
Keywords:
Androgen receptorFibronectinFibronectin type III domain containing 1MiR-1207-3pProstate cancer

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