Related Experiment Video

Updated: Mar 14, 2026

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
08:54

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

Published on: July 20, 2014

14.5K

Proto-Oncogenic Src Phosphorylates EB1 to Regulate the Microtubule-Focal Adhesion Crosstalk and Stimulate Cell

Yijun Zhang1, Youguang Luo1, Rui Lyu1

  • 1State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.

Theranostics
|October 5, 2016
PubMed

Insights

The proto-oncogenic protein Src regulates cell migration by phosphorylating end-binding protein 1 (EB1), impacting microtubule dynamics and focal adhesions. This discovery clarifies molecular mechanisms driving tumor metastasis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Cell migration is crucial for tumor progression and metastasis.
  • The crosstalk between microtubules (MTs) and focal adhesions (FAs) is vital for cell migration.
  • Molecular mechanisms regulating MT-FA crosstalk remain largely unknown.

Purpose of the Study:

  • To identify key molecular players regulating MT-FA crosstalk.
  • To elucidate the role of the proto-oncogenic protein Src in this process.
  • To investigate the interaction between Src and end-binding protein 1 (EB1).

Main Methods:

  • Investigated Src interaction and phosphorylation of EB1 in vitro and in cells.
  • Utilized systematic mutagenesis to identify the specific phosphorylation site on EB1 (Y247).
  • Examined the localization of Src and phosphorylated EB1.
  • Assessed the impact of EB1 phosphorylation on its interactions with other plus-end tracking proteins (+TIPs) and MT dynamics.

Main Results:

  • Src directly interacts with and phosphorylates EB1 at tyrosine-247 (Y247).
  • Both activated Src and Y247-phosphorylated EB1 localize to centrosomes and FAs.
  • Src-mediated EB1 phosphorylation reduces its binding to other +TIPs (APC, MCAK).
  • EB1 phosphorylation at Y247 accelerates MT catastrophe and enhances cell migration.

Conclusions:

  • The Src-EB1 signaling axis is a critical regulator of MT-FA crosstalk.
  • Src-mediated phosphorylation of EB1 promotes cell migration by altering MT dynamics.
  • This pathway represents a potential therapeutic target for inhibiting tumor metastasis.
Keywords:
EB1Srccell migration.focal adhesionmicrotubulephosphorylation

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

890
Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

7.1K

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.0K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.1K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.7K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K