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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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Multi-parameter analysis using photovoltaic cell-based optofluidic cytometer.

Chien-Shun Yan1, Yao-Nan Wang1

  • 1Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.

Biomedical Optics Express
|October 5, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a low-cost optofluidic cytometer that simultaneously measures side scattered (SSC), extinction (EXT), and fluorescence (FL) signals. The device demonstrates high accuracy in detecting various bead sizes, offering a versatile solution for point-of-care applications.

Keywords:
(120.5820) Scattering measurements(130.3120) Integrated optics devices(170.0170) Medical optics and biotechnology(230.3990) Micro-optical devices(300.2530) Fluorescence, laser-induced

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Area of Science:

  • Optofluidics
  • Biomedical Engineering
  • Photonics

Background:

  • Traditional flow cytometry can be expensive and complex.
  • There is a need for accessible, multi-parameter cell analysis tools.

Purpose of the Study:

  • To develop and demonstrate a low-cost, multi-parameter optofluidic cytometer.
  • To enable simultaneous detection of side scattered (SSC), extinction (EXT), and fluorescence (FL) signals.
  • To validate the device's performance for point-of-care (PoC) applications.

Main Methods:

  • Fabrication of an optofluidic cytometer on a polydimethylsiloxane (PDMS) substrate.
  • Utilizing two commercial photovoltaic cells and an avalanche photodetector for signal detection.
  • Employing a free-space light transmission technique without on-chip optical waveguides.
  • Detection of fluorescent-labeled and label-free polystyrene beads (3-10 μm).

Main Results:

  • Simultaneous detection of SSC, EXT, and FL signals was achieved.
  • High detection accuracy was demonstrated for single and mixed bead populations.
  • Performance was validated against a commercial flow cytometer.
  • Successful detection of beads with sizes ranging from 3 μm to 10 μm.

Conclusions:

  • The proposed optofluidic cytometer offers a versatile, straightforward, and low-cost solution.
  • The device shows significant potential for various point-of-care (PoC) cytometry applications.
  • The free-space light transmission technique eliminates the need for complex on-chip waveguides.