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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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Visualization analysis of CRISPR/Cas9 gene editing technology studies.

Quan-Sheng Du1, Jie Cui2, Chun-Jie Zhang3

  • 1Department of Life Sciences, National Natural Science Foundation of China, Beijing 100085, China.

Journal of Zhejiang University. Science. B
|October 6, 2016
PubMed
Summary

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system offers bacterial defense. This study maps CRISPR/Cas9 gene editing research hotspots from 2002-2015 using knowledge mapping.

Keywords:
CRISPR/Cas9CiteSpaceVVisualization analysis

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Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system functions as an adaptive immune mechanism in bacteria and archaea.
  • This system effectively defends against invading viruses and foreign genetic material like plasmids.

Purpose of the Study:

  • To analyze the research landscape of CRISPR/Cas9 gene editing technology.
  • To identify emerging trends and key research areas within the field.

Main Methods:

  • Bibliometric analysis of scientific literature.
  • Utilizing the Web of Science database for data collection (2002-2015).
  • Employing CiteSpaceV software for co-citation analysis and knowledge mapping.

Main Results:

  • Identification of prominent research hotspots in CRISPR/Cas9 gene editing.
  • Mapping the evolution and frontiers of CRISPR/Cas9 research over the specified period.
  • Visual representation of the intellectual structure and development of the field.

Conclusions:

  • The study provides a comprehensive overview of CRISPR/Cas9 gene editing research.
  • Knowledge mapping reveals the dynamic nature and key developmental trajectories of this technology.
  • Understanding research hotspots aids in directing future scientific inquiry and innovation.