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Cells of the Adaptive Immune Response01:23

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The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
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The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
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Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
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Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
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Deciphering the Innate Lymphoid Cell Transcriptional Program.

Cyril Seillet1, Lisa A Mielke1, Daniela B Amann-Zalcenstein1

  • 1The Walter and Eliza Hall Institute of Medical Research and Department of Medical Biology, University of Melbourne, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia.

Cell Reports
|October 6, 2016
PubMed
Summary
This summary is machine-generated.

Understanding the temporal gene expression program is key for innate lymphoid cell (ILC) progenitor development. This study reveals the critical timing of transcription factors NFIL3, ID2, and TCF-1 for ILC lineage commitment.

Keywords:
developmentimmunityinnateinnate lymphoid cellstranscription factors

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Area of Science:

  • Immunology
  • Developmental Biology
  • Transcriptional Regulation

Background:

  • Innate lymphoid cells (ILCs) are crucial for immune surveillance at mucosal surfaces.
  • The development of distinct ILC lineages from a common progenitor involves pre-committed progenitors.
  • The precise temporal control of gene expression guiding progenitor emergence remains largely unknown.

Purpose of the Study:

  • To investigate the temporal gene expression dynamics during innate lymphoid cell (ILC) progenitor development.
  • To identify key transcription factors and regulatory events governing ILC lineage commitment.
  • To elucidate the transcriptional program underlying ILC progenitor differentiation.

Main Methods:

  • Global transcriptional mapping of gene expression in distinct ILC progenitors.
  • Analysis of the expression patterns of transcription factors NFIL3, ID2, and TCF-1.
  • Identification of specific markers like PD-1 in ILC progenitors.

Main Results:

  • PD-1 was identified as specifically expressed in PLZF+ ILC progenitors.
  • The timing and order of NFIL3, ID2, and TCF-1 expression were found to be critical for ILC development.
  • Transient NFIL3 expression preceding ID2 and TCF-1 was sufficient for ILC lineage commitment.

Conclusions:

  • A specific temporal gene expression program dictates the commitment of progenitors to the ILC lineage.
  • The findings expand the understanding of the core transcriptional network regulating ILC development.
  • This study identifies potential novel regulators involved in ILC progenitor differentiation.