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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Published on: March 24, 2012
Mass spectrometry-based assay for the rapid detection of thiol-containing natural products
Stacy L Capehart1, Erin E Carlson2
1Department of Chemistry, University of Minnesota, 207 Pleasant St., SE Minneapolis, 55455-0431, USA. carlsone@umn.edu.
Abstract:
Natural products are privileged scaffolds due to their high propensity to possess bioactivity. To expedite discovery of thiol-containing compounds, we devised a selective solid-supported reagent for their immobilization, followed by cleavage of a photocleavable linker to yield stable natural product conjugates for direct detection by mass spectrometry. Importantly, the natural products can also be tracelessly released to yield the native structures for chemical and biological evaluation.
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