Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

7.2K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physics-based parameterisation framework for basal melting in ice-ocean boundary layers over dynamically stable pycnoclines.

Communications earth & environment·2025
Same author

Isolation of a novel microplastic-degrading bacterial strain: a promising agent for low-density polyethylene remediation.

Chemosphere·2025
Same author

Nurses' attitudes, behaviours, and enablers of intravenous to oral switching (IVOS) of antibiotics: a mixed-methods survey of nursing staff in secondary care hospitals across the Midlands region of England.

The Journal of hospital infection·2024
Same author

Increased warm water intrusions could cause mass loss in East Antarctica during the next 200 years.

Nature communications·2023
Same author

Socio-economic and ethnic disparities in childhood cancer survival, Yorkshire, UK.

British journal of cancer·2023
Same author

A High-End Estimate of Sea Level Rise for Practitioners.

Earth's future·2023
Same journal

A tri-axis optomechanical accelerometer with plasmonic MIM waveguide and structural direction-dependent optical signatures.

Scientific reports·2026
Same journal

Holographic leaky-wave antennas with independently controlled multiple counter-rotating vortex beams.

Scientific reports·2026
Same journal

Differential associations of longitudinal hearing and vision trajectories with dementia and mild cognitive impairment in older adults.

Scientific reports·2026
Same journal

Abdominal obesity and leisure-time sedentary behavior in relation to gastroesophageal reflux disease risk: a prospective cohort study from the UK Biobank.

Scientific reports·2026
Same journal

Effect of nitrogen-rich COF incorporation on the structure and separation performance of polyamide nanofiltration membranes.

Scientific reports·2026
Same journal

Withanolide A inhibits hIAPP aggregation: An In silico, biophysical, and drosophila-based In vivo validation.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 13, 2026

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
05:30

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Published on: October 8, 2008

25.9K

Cleaning patch-clamp pipettes for immediate reuse.

I Kolb1, W A Stoy1, E B Rousseau2

  • 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, 30332 USA.

Scientific Reports
|October 12, 2016
PubMed
Summary
This summary is machine-generated.

Automated pipette cleaning allows rapid reuse in patch-clamp electrophysiology, significantly increasing throughput for neuronal studies. This innovation enables sequential recordings without manual pipette replacement, advancing large-scale brain mapping.

More Related Videos

Pressure-polishing Pipettes for Improved Patch-clamp Recording
05:12

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Published on: October 22, 2008

13.4K
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.4K

Related Experiment Videos

Last Updated: Mar 13, 2026

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
05:30

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Published on: October 8, 2008

25.9K
Pressure-polishing Pipettes for Improved Patch-clamp Recording
05:12

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Published on: October 22, 2008

13.4K
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.4K

Area of Science:

  • Neuroscience
  • Electrophysiology
  • Biotechnology

Background:

  • Patch-clamp recording is crucial for high-resolution single-cell analysis but is limited by low throughput due to manual pipette replacement.
  • Current methods are laborious and impractical for large-scale neuronal characterization, hindering comprehensive brain mapping.
  • The manual replacement of patch-clamp pipettes is a significant bottleneck for high-throughput applications.

Purpose of the Study:

  • To develop a fast, automated method for cleaning and reusing glass pipette electrodes for patch-clamp recordings.
  • To overcome the throughput limitations of traditional patch-clamp techniques.
  • To enable sequential patch-clamp recordings without manual intervention.

Main Methods:

  • Developed an automated cleaning protocol involving immersion in Alconox detergent followed by rinsing.
  • Tested pipette reuse across various experimental preparations, including cell cultures, brain slices, and in vivo recordings.
  • Validated signal fidelity and ion channel pharmacology after pipette cleaning and reuse.
  • Engineered the first robot for automated sequential patch-clamp recordings.

Main Results:

  • Pipettes were successfully reused up to 10 times with no degradation in signal fidelity.
  • The cleaning method was effective in diverse biological preparations, from cell lines to in vivo neurons.
  • Residual Alconox did not impact ion channel pharmacology.
  • The developed robot successfully performed sequential patch-clamp recordings autonomously.

Conclusions:

  • Automated pipette cleaning provides a simple, rapid solution to enhance patch-clamp recording throughput.
  • This method significantly reduces labor and enables large-scale neuronal characterization and brain mapping.
  • The developed robotic system represents a breakthrough for automated, high-throughput electrophysiology.