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Updated: Mar 13, 2026

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Isolation of Protein Storage Vacuoles and Their Membranes
Tomoo Shimada1, Ikuko Hara-Nishimura2
1Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyoku, Kyoto, 606-8502, Japan.
Abstract:
Protein-storage vacuoles (PSVs) are specialized vacuoles that sequester large amounts of storage proteins. During seed development, PSVs are formed de novo and/or from preexisting lytic vacuoles. Seed PSVs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. In this chapter, we introduce easy methods for isolation of PSVs and their membranes from pumpkin seeds. These methods facilitate the identification and characterization of PSV components.
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