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Updated: Nov 25, 2025

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
Published on: June 15, 2018
Isolation of Plasma Membrane and Plasma Membrane Microdomains
Anzu Minami1, Daisuke Takahashi2, Yukio Kawamura2
1Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
Abstract:
The plasma membrane surrounds the cytoplasm of a cell and functions as a barrier to separate the intracellular compartment from the extracellular environment. Protein and lipid components distribute nonuniformly and the components form clusters with various functions in the plasma membrane. These clusters are called as "microdomains." In plant cells, microdomains have been studied extensively because they play important roles in biotic/abiotic stress responses, cellular trafficking, and cell wall metabolism. Here we describe a standard protocol for the isolation of the plasma membrane and microdomains from plant cells, Arabidopsis and oat.
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