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Related Concept Videos

Instrument Calibration01:12

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Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
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A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
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Spectral phase-based automatic calibration scheme for swept source-based optical coherence tomography systems.

K M Ratheesh1, L K Seah, V M Murukeshan

  • 1Center for Optical & Laser Engineering, School of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.

Physics in Medicine and Biology
|October 15, 2016
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Summary

This study introduces a novel real-time automatic calibration method for swept-source optical coherence tomography (SS-OCT) systems. The new scheme enhances imaging accuracy and speed without additional hardware, improving Fourier-domain optical coherence tomography (FD-OCT) applications.

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Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Metrology

Background:

  • Fourier-domain optical coherence tomography (FD-OCT) systems offer high-resolution imaging crucial for applications like microsurgery.
  • Existing automatic calibration methods in FD-OCT suffer from limitations in accuracy and speed due to approximations and iterative processes.

Purpose of the Study:

  • To develop a new real-time automatic calibration scheme for swept-source optical coherence tomography (SS-OCT) systems.
  • To improve the accuracy and speed of calibration without increasing computational or hardware complexity.

Main Methods:

  • A novel real-time automatic calibration scheme utilizing the spectral component of sample surface reflection as the calibration signal.
  • Determining the spectral phase function of the frequency-swept laser source and applying phase linearization through normalization and rescaling.
  • Estimating fractional-time indices for resampling OCT signals, enabling precise calibration regardless of sample topography or scanning variations.

Main Results:

  • The proposed method successfully calibrates SS-OCT systems in real-time during scanning operations.
  • Calibration is achieved without requiring auxiliary interferometers or additional acquisition channels.
  • Demonstrated high-performance calibration in terms of axial resolution and sensitivity on an in-house SS-OCT system.

Conclusions:

  • The developed automatic calibration scheme offers a significant advancement for SS-OCT systems.
  • It provides accurate and fast calibration, enhancing the utility of OCT in complex imaging scenarios.
  • The method is hardware-efficient and computationally straightforward, making it broadly applicable.