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Release from optimal compressive force suppresses osteoclast differentiation.
Masaaki Ikeda1, Yoshitaka Yoshimura2, Takashi Kikuiri3
1Department of Orthodontics, Hokkaido University Graduate School of Dental Medicine, Sapporo, Hokkaido 060‑8586, Japan.
Molecular Medicine Reports
|October 26, 2016
Summary
Releasing compressive force during orthodontic treatment suppresses osteoclast activity, potentially impacting tooth movement. This finding offers insights for refining orthodontic therapies.
Area of Science:
- Biomedical Engineering
- Orthodontics
- Cell Biology
Background:
- Bone remodeling is crucial for orthodontic tooth movement.
- Mechanical forces influence osteoclast differentiation and fusion.
- Release from tensile force promotes osteoclast differentiation, but effects of releasing compressive force are unclear.
Purpose of the Study:
- To investigate the impact of releasing compressive force on osteoclasts.
- To evaluate osteoclast differentiation and fusion following force release.
Main Methods:
- RAW264.7 cells were used to model osteoclasts.
- Osteoclast number was quantified via tartrate-resistant acid phosphatase (TRAP) staining.
- Gene expression related to osteoclastogenesis was analyzed using RT-qPCR.
Main Results:
- Optimal compressive force application increased osteoclast numbers.
- Release from compressive force suppressed osteoclast differentiation and fusion.
- Key osteoclast marker genes, including NFATc1, TRAP, RANK, and OC-STAMP, showed significant inhibition post-force release.
Conclusions:
- Release from optimal compressive force inhibits osteoclast differentiation and fusion.
- These findings suggest a mechanism relevant to orthodontic tooth movement.
- Understanding these effects could aid in developing improved orthodontic treatments.
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