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A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
Lucie Pinasseau1, Arnaud Verbaere2, Maryline Roques3,4,5
1Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. pinassea@supagro.inra.fr.
Molecules (Basel, Switzerland)
|October 25, 2016
Summary
A new method using UHPLC-QqQ-MS rapidly analyzes grape skin flavan-3-ols and proanthocyanidins. This technique is suitable for large-scale studies and may reveal genetic factors influencing grape drought resistance.
Area of Science:
- Analytical Chemistry
- Plant Science
- Metabolomics
Background:
- Grape skin proanthocyanidins (flavan-3-ols) are complex polyphenols.
- Understanding their composition is crucial for grape quality and stress response.
- Previous analytical methods were time-consuming and less sensitive.
Purpose of the Study:
- To develop a rapid, sensitive, and selective method for analyzing grape skin flavan-3-ols.
- To quantify constitutive units of proanthocyanidins, including trace compounds.
- To apply the method to large-scale grape metabolomics studies.
Main Methods:
- Ultra High Performance Liquid Chromatography coupled with Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ-MS).
- Acid-catalyzed depolymerization in the presence of phloroglucinol (phloroglucinolysis).
- Detection using Multiple Reaction Monitoring (MRM) mode for specific compound identification.
Main Results:
- A robust UHPLC-QqQ-MS method was established for flavan-3-ol and proanthocyanidin analysis.
- The method achieved high throughput (6 samples/hour) and detected trace compounds.
- Analysis of 214 grape skin samples from 107 cultivars confirmed method robustness.
Conclusions:
- The developed method is suitable for high-throughput, large-scale metabolomics.
- Tannin composition analysis is relevant for investigating genetic bases of grape drought response.
- This advancement facilitates deeper understanding of grape physiology under environmental stress.
