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High-performance liquid chromatography of amino acids, peptides, proteins and polynucleotides. XCIV. Solid-phase
M Guthridge1, J Bertolini, M T Hearn
1Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Journal of Chromatography
|August 4, 1989
Abstract:
The ability of synthetic oligonucleotides to specifically hybridize to a complementary oligonucleotide immobilized on an anionic stationary phase has been investigated. A sigmoidal melting curve was obtained when oligonucleotide duplex formation on the column was plotted against hybridization stringency over the ionic strength range 0.2-0.42 M. These studies confirm a rapid method for determining the relative melting temperature of hybridized oligonucleotide complexes and provide a basis for the selection of stringency conditions optimal for various synthetic oligonucleotide probes.