Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.9K
Computed Tomography01:10

Computed Tomography

9.3K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
9.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.7K
X-ray Imaging01:24

X-ray Imaging

10.9K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dianionic Tetraalkynylmagnesate Complexes: A Strategy for Propargyl Amine Synthesis.

Organic letters·2026
Same author

Redox Mechanisms of Silica-Supported Ni Particles: An X-Ray Absorption Fine Structure Investigation.

Materials (Basel, Switzerland)·2026
Same author

A DFT Study on the Mechanism of Selective Formation of Substituted Azepines From 1-Azabutadienes and Cyclopropanes.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Facile Synthesis of α-Silyl Alcohols via Addition of Trimethylsilyllithium to Carbonyl Compounds.

Chemical & pharmaceutical bulletin·2026
Same author

Precise 3D structure determination of Cu single atoms on an α-Al<sub>2</sub>O<sub>3</sub>(0001) surface by polarization-dependent total reflection fluorescence X-ray absorption fine structure and first-principles calculations.

Physical chemistry chemical physics : PCCP·2026
Same author

Alpha-ketoglutarate supplementation improves hyperglycemia and attenuates the decrease in GLUT4 and PGC-1α proteins in adipose tissue of streptozotocin-high-fat diet-induced diabetic mice.

Journal of nutritional science·2026

Related Experiment Video

Updated: Mar 13, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.7K

X-ray analyzer-based phase-contrast computed laminography.

Keiichi Hirano1, Yumiko Takahashi1, Kazuyuki Hyodo1

  • 1Institute of Materials Structure Science, High Energy Accelerator Research Organization, 1-1, Oho, Tsukuba, Ibaraki 305-0801, Japan.

Journal of Synchrotron Radiation
|October 28, 2016
PubMed
Summary

This study combines X-ray phase-contrast imaging with computed laminography for advanced material analysis. The novel technique successfully generated phase-contrast sectional images of plastic beads, demonstrating its potential for weak absorption contrast imaging.

Keywords:
X-ray phase-contrast imagingcomputed laminographyimage processing

More Related Videos

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.4K
Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph
05:32

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph

Published on: February 21, 2025

763

Related Experiment Videos

Last Updated: Mar 13, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.7K
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.4K
Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph
05:32

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph

Published on: February 21, 2025

763

Area of Science:

  • Physics
  • Materials Science
  • Imaging Technology

Background:

  • Imaging weakly absorbing materials presents challenges for conventional methods.
  • Phase-contrast imaging offers enhanced sensitivity to material variations.

Purpose of the Study:

  • To develop and validate a combined X-ray phase-contrast imaging and computed laminography technique.
  • To enable multi-contrast imaging of extended flat specimens.

Main Methods:

  • Utilized X-ray analyzer-based phase-contrast imaging with computed laminography.
  • Employed a nondispersive (+, -) diffraction geometry with specific crystal optics.
  • Developed a generalized algorithm for multi-contrast image calculation and laminographic reconstruction.

Main Results:

  • Successfully obtained phase-contrast sectional images of plastic beads at a wavelength of 0.0733 nm.
  • Demonstrated the feasibility of the combined imaging method at the Photon Factory beamline.
  • Identified circular artifacts limiting the field of view to approximately 6 mm.

Conclusions:

  • The integrated X-ray phase-contrast imaging and computed laminography method is feasible for analyzing weak absorption contrast specimens.
  • Further optimization is needed to mitigate artifacts and expand the field of view for practical applications.