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A bubble- and clogging-free microfluidic particle separation platform with multi-filtration.

Yinuo Cheng1, Yue Wang1, Zengshuai Ma1

  • 1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, China. wwh@mail.tsinghua.edu.cn xyye@mail.tsinghua.edu.cn.

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Summary

This study presents a novel microfluidic platform for bubble-free and clogging-free particle separation. The device utilizes a bidirectional micropump and specialized membranes for efficient and reliable separation of particles and cells, even from whole blood.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Separation Science

Background:

  • Microfiltration is effective for particle separation but suffers from clogging and bubble formation.
  • These issues limit the reliability and throughput of microfluidic separation devices.

Purpose of the Study:

  • To develop a microfluidic platform that overcomes clogging and bubble formation for high-throughput particle separation.
  • To demonstrate the platform's efficacy in separating microbeads and blood cells.

Main Methods:

  • Integrated bidirectional micropump for forward and reverse flushing of the filtration membrane.
  • Hydrophilic microporous filtration membrane for particle retention.
  • Hydrophobic porous degassing membrane to remove air bubbles.

Main Results:

  • Achieved 2857-fold enrichment and 89.8% recovery of 10 μm microbeads with 99.9% removal of 2 μm microbeads.
  • Successfully separated white blood cells from whole blood with 396-fold enrichment and 70.6% recovery at 39.1 μl min⁻¹ throughput.
  • Demonstrated clog-free and bubble-free operation over multiple cycles.

Conclusions:

  • The developed microfluidic platform offers a robust solution for bubble-free and clogging-free particle and cell separation.
  • High throughput and efficiency make the platform suitable for various biomedical applications, including cell separation from blood.