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Single-Multiplex Detection of Organ Injury Biomarkers using SPRi based Nano-Immunosensor.

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  • 1Department of Nanoscience, University of North Carolina at Greensboro, Greensboro, NC 27455, USA.

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Summary

This study introduces a novel biosensor for simultaneously detecting kidney injury molecule (KIM-1) and high mobility group box-1 (HMGB-1). The platform offers real-time, label-free quantification, aiding early diagnosis of organ dysfunction.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Clinical Diagnostics

Background:

  • Early assessment of multiple organ dysfunction is crucial for timely treatment and preventing irreversible damage.
  • Current diagnostic methods may lack the sensitivity or speed required for real-time monitoring.

Purpose of the Study:

  • To develop a real-time, label-free, and multiplex nanoenhanced surface plasmon resonance imaging (SPRi) platform.
  • To quantitatively assess two key biomarkers: kidney injury molecule (KIM-1) and high mobility group box-1 (HMGB-1) simultaneously.

Main Methods:

  • Site-specific antibody immobilization on a solid surface to maintain biological activity.
  • Development of a blocked sensor surface to minimize non-specific adsorption for accurate singleplex measurements.
  • Implementation of a sandwich assay utilizing functionalized quantum dots (NanoEnhancers) as signal amplifiers.

Main Results:

  • Achieved ultra-sensitive detection with a sensitivity level of 5 pg/mL for both KIM-1 and HMGB-1 in buffer.
  • Demonstrated simultaneous, quantitative assessment of two distinct biomarkers.
  • Successfully minimized non-specific adsorption through a well-blocked sensor surface design.

Conclusions:

  • The developed nanoenhanced SPRi platform enables simultaneous, ultra-sensitive detection of KIM-1 and HMGB-1.
  • This technology holds significant potential for early clinical assessment of organ dysfunction.
  • The platform can be extended to detect other emerging biomarkers in complex biological matrices.