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Updated: Mar 12, 2026
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Flavin-mediated photo-oxidation for the detection of mitochondrial flavins
Dong-Nam Lee1, Eunjin Kim2, Jae Hong Lee3
1Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 08826, Republic of Korea. jihong@snu.ac.kr.
Abstract:
We report a new approach for the detection of mitochondrial flavins through photo-oxidation of a probe molecule. Probe 1 showed high brightness (ε × Φf = 6.50 × 103 M-1 cm-1) at long wavelengths (maximum emission wavelength, λmax = 600 nm) upon photo-oxidation, assisted by the strong electron accepting ability of the isoalloxazine moiety in flavins. Probe 1 also exhibited high selectivity for flavins over various biological oxidants, remarkable photo-stability, and mitochondrial localization.
