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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Mar 12, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
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Zebrafish Genome Engineering Using the CRISPR-Cas9 System.

Mingyu Li1, Liyuan Zhao2, Patrick S Page-McCaw3

  • 1School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China.

Trends in Genetics : TIG
|November 13, 2016
PubMed
Summary
This summary is machine-generated.

Scientists can now precisely edit vertebrate DNA using CRISPR-Cas9 technology. This gene editing tool, applied in zebrafish, enables targeted gene function analysis and genome engineering.

Keywords:
CRISPR–Cas9genetic screengenome editingknock-inknockoutzebrafish

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Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Geneticists aim to manipulate vertebrate genomes for gene function analysis.
  • The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system offers precise genome editing capabilities.
  • CRISPR-Cas9 generates double-strand breaks (DSBs) at specific DNA loci.

Purpose of the Study:

  • To discuss the development and application of CRISPR-Cas9 in the zebrafish model.
  • To explain how CRISPR-Cas9 facilitates genome engineering in vertebrates.
  • To highlight the utility of CRISPR-Cas9 for analyzing gene function.

Main Methods:

  • Utilizing CRISPR-Cas9 endonuclease to target specific genomic loci.
  • Inducing double-strand breaks (DSBs) at targeted sites.
  • Employing homology-directed repair with a supplied template for engineered alleles.

Main Results:

  • CRISPR-Cas9 enables targeted gene modification in zebrafish.
  • Endogenous repair mechanisms (insertions/deletions) or engineered alleles are generated at DSB sites.
  • Facilitates the analysis of gene function through precise genetic alterations.

Conclusions:

  • CRISPR-Cas9 is a powerful tool for vertebrate genome engineering.
  • Its adoption in zebrafish accelerates research in developmental biology and genetics.
  • Enables efficient manipulation of gene function for scientific discovery.