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Updated: Mar 12, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
The Molecular Blueprint of a Fungus by Next-Generation Sequencing (NGS)
Christian Grumaz1, Philipp Kirstahler1, Kai Sohn2,3
1Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany.
Abstract:
Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation.
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