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Development of Interleukin-2 Loaded Chitosan-Based Nanogels Using Artificial Neural Networks and Investigating the
Canan Aslan1,2, Nevin Çelebi3, I Tuncer Değim1
1Department of Pharmaceutical Technology, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.
AAPS Pharmscitech
|November 18, 2016
Summary
This study developed novel nanogels loaded with interleukin-2 (IL-2) for wound healing. The chitosan-TPP nanogels effectively improved wound healing in rats by reducing oxidative stress markers.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Nanotechnology
Background:
- Wound healing is a complex biological process that requires effective therapeutic strategies.
- Interleukin-2 (IL-2) has immunomodulatory properties beneficial for tissue repair.
- Developing suitable delivery systems for IL-2 is crucial for enhancing its therapeutic efficacy.
Purpose of the Study:
- To develop and characterize recombinant human interleukin-2 (rh-IL-2) loaded chitosan-based nanogels.
- To evaluate the wound healing potential of these nanogels in a rat incision model.
Main Methods:
- Nanogels were prepared using chitosan with either bovine serum albumin (BSA) or tripolyphosphate (TPP).
- Characterization included particle size, zeta potential, polydispersity index, and morphology (SEM, AFM).
- IL-2 loading capacity, in vitro release, and wound healing effects (MDA and GSH levels) were assessed.
Main Results:
- Both BSA-chitosan and chitosan-TPP nanogels exhibited 100% IL-2 loading capacity.
- IL-2 release was slow after an initial burst.
- Chitosan-TPP nanogels, when loaded with IL-2, significantly reduced malondialdehyde (MDA) and increased glutathione (GSH) levels in wound tissues, indicating improved healing.
Conclusions:
- Chitosan-based nanogels are effective carriers for rh-IL-2.
- IL-2 loaded chitosan-TPP nanogels demonstrate significant potential for promoting wound healing by modulating oxidative stress.
