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Reusable hydroxyapatite nanocrystal sensors for protein adsorption
Motohiro Tagaya1, Toshiyuki Ikoma1, Nobutaka Hanagata2
1Biomaterials Center, National Institute for Materials Science, Tsukuba, Ibaraki 305-0047, Japan; Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Tokyo 152-8550, Japan.
Science and Technology of Advanced Materials
|November 24, 2016
Summary
The APM/UV treatment effectively removes proteins from hydroxyapatite (HAp) sensors, enabling repeatable protein adsorption studies. This method is crucial for reliable biosensor development and surface science research.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Nanotechnology
Background:
- Hydroxyapatite (HAp) nanocrystals are vital in biosensing applications.
- Understanding protein adsorption and removal is key for sensor reusability.
- Previous methods for cleaning HAp sensors were not fully evaluated for repeatability.
Purpose of the Study:
- To investigate the repeatability of protein adsorption and removal on HAp nanocrystal sensors.
- To compare the effectiveness of different cleaning methods for HAp sensors.
- To identify the optimal method for regenerating HAp sensors for repeated use.
Main Methods:
- Fourier transform infrared (FTIR) spectroscopy and quartz crystal microbalance with dissipation (QCM-D) were used.
- HAp nanocrystals were deposited onto gold-coated quartz sensors via electrophoretic deposition.
- Proteins (fibrinogen, fetal bovine serum) were adsorbed and then removed using ammonia/hydrogen peroxide mixture (APM), UV light, APM/UV, UV/APM, and sodium dodecyl sulfate (SDS) treatments.
Main Results:
- FTIR showed APM and SDS treatments left residual proteins, while UV and APM/UV treatments removed them effectively.
- QCM-D revealed fibrinogen adsorption changes due to surface wettability shifts after initial removal cycles.
- SDS was ineffective for protein removal; APM or UV treatments altered sensor frequency shifts; UV/APM minimized frequency shifts and reduced dissipation shifts.
Conclusions:
- The APM/UV treatment is the most effective method for regenerating HAp sensors.
- This treatment ensures reproducible protein adsorption behavior on HAp sensors.
- The findings are critical for developing reliable and reusable HAp-based biosensing platforms.
