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A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii
1Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Nucleic Acids Research
|July 11, 1989
Abstract:
The coding region for the Pneumocystis carinii small subunit rRNA (16S-like rRNA) contains 390 extra base pairs in a region that is highly conserved in other eukaryotic rRNA genes. The sequence has all the elements characteristic of group I self-splicing introns and interrupts the phylogenetically conserved helix proximal to the 3' terminus of the 16S-like rRNA. In vitro transcripts containing the putative group I intron were capable of undergoing a "self-splicing" reaction which required the presence of guanine nucleotide.