Osteoblast response to the surface topography of hydroxyapatite two-dimensional films

In the current study, three hydroxyapatite two-dimensional films with similar wettability but different topological roughness have been prepared through a facile one-step method using hydrated polylactic acid discs as substrates. The results indicated that the protein adsorption capabilities of the hydroxyapatite two-dimensional films and the proliferation of osteoblast cells on the as-prepared films increased obviously with the increase of the films' roughness. These reveal that the cellular responses to two-dimensional materials can be efficiently tuned by easily adjusting the topological roughness. This finding affords an efficient strategy to regulate the cellular response to bioinorganic two-dimensional materials and significantly expanded their application potentials in tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 991-999, 2017.