Related Experiment Video
Updated: Mar 11, 2026
11:00
Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
11.7K
Atmospheric pressure thermal ionization ion source for peptide analysis.
Eugenii N Kukaev1, Alexey S Kononikhin2, Natalia L Starodubtseva3
1Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russian Federation and Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russian Federation.
European Journal of Mass Spectrometry (Chichester, England)
|December 1, 2016
Summary
A new atmospheric pressure thermal ionization (APTI) ion source effectively analyzes liquid samples, including peptides. This method shows potential for biological sample analysis with minimal sample preparation.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Biochemistry
Background:
- Liquid sample analysis often requires complex pre-treatment.
- Developing efficient ionization sources is crucial for mass spectrometry.
Purpose of the Study:
- To develop and demonstrate a novel atmospheric pressure thermal ionization (APTI) ion source for liquid samples.
- To evaluate the performance of APTI for peptide analysis.
Main Methods:
- Development of an APTI ion source utilizing microalloyed molybdenum as a thermal ion emitter.
- Testing two configurations: hot wire-assisted and hot surface-assisted ionization.
- Analysis of peptide samples.
Main Results:
- Successful generation of multiply-charged ions from peptides using APTI.
- Observation of peptide fragmentation during thermal ionization.
- Demonstration of peptide identification potential through fragmentation patterns.
Conclusions:
- The novel APTI ion source is feasible for liquid sample analysis, particularly peptides.
- Peptide fragmentation in APTI can be leveraged for identification.
- The method holds promise for analyzing biological samples with reduced pre-treatment.
Related Concept Videos
01:19Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
2.4K
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
2.4K
01:21Chemical Ionization (CI) Mass Spectrometry
1.6K
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
1.6K
01:33Peptide Identification Using Tandem Mass Spectrometry
8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K
01:12Electrospray Ionization (ESI) Mass Spectrometry
2.6K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.6K
01:19Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle
2.1K
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
2.1K
01:19MALDI-TOF Mass Spectrometry
7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K