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MiRNA-543 promotes osteosarcoma cell proliferation and glycolysis by partially suppressing PRMT9 and stabilizing

Heng Zhang1, Xiaofeng Guo2, Xing Feng3

  • 1Department of Orthopedics, Renhe Hospital, China Three Gorges University, Yichang, Hubei, China.

Oncotarget
|December 3, 2016
PubMed

Insights

MicroRNA-543 (miR-543) promotes osteosarcoma (OS) growth by inhibiting PRMT9, affecting cell metabolism. Targeting the miR-543/PRMT9/HIF-1α axis offers a potential therapeutic strategy for osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a prevalent and aggressive bone cancer, particularly in adolescents.
  • MicroRNAs are crucial regulators in cancer development, presenting potential therapeutic targets.

Purpose of the Study:

  • To investigate the role of miR-543 in osteosarcoma (OS) development.
  • To explore the relationship between miR-543 and PRMT9 in OS.
  • To elucidate the therapeutic potential of targeting the miR-543/PRMT9 axis in OS.

Main Methods:

  • Analysis of miR-543 and PRMT9 expression in OS tissues and cell lines.
  • Bioinformatics prediction and luciferase-reporter assay to confirm miR-543 targeting of PRMT9.
  • In vitro and in vivo experiments to assess the impact of miR-543 on OS cell proliferation.
  • Investigation of the effects of miR-543 and PRMT9 on cellular oxidative phosphorylation, glycolysis, and HIF-1α stability.

Main Results:

  • miR-543 levels were elevated, while PRMT9 levels were decreased in OS tissues.
  • miR-543 directly targets and inhibits PRMT9 expression in OS cells.
  • miR-543 promotes OS cell proliferation both in vitro and in vivo.
  • miR-543 dysregulates cellular metabolism by inhibiting oxidative phosphorylation and affecting glycolysis and HIF-1α stability.

Conclusions:

  • The miR-543/PRMT9/HIF-1α axis plays a significant role in osteosarcoma development.
  • Targeting the glycolytic pathway via the miR-543/PRMT9/HIF-1α axis presents a promising therapeutic strategy for osteosarcoma.
Keywords:
HIF-1αPRMT9glycolysismiRNA-543osteosarcoma

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