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Serial Epithelial Lining Fluid Collection Using Bronchoscopic Microsampling in a Canine Lung Transplantation Model
Kana Oiwa, Mitsutomo Kohno1, Ryo Hashimoto
1Division of General Thoracic Surgery, Department of Surgery Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. kohno@1993.jukuin.keio.ac.jp.
The Tokai Journal of Experimental and Clinical Medicine
|December 19, 2016
Summary
Bronchoscopic microsampling (BMS) safely collects epithelial lining fluid (ELF) for serial analysis after lung transplants. This method detected elevated TNF-α, indicating lung injury, and is a promising tool for monitoring pulmonary function.
Area of Science:
- Pulmonary Medicine
- Transplantation Immunology
- Respiratory Physiology
Background:
- Bronchoalveolar lavage (BAL) is invasive.
- Bronchoscopic microsampling (BMS) offers a less invasive method to collect epithelial lining fluid (ELF).
- Evaluating BMS for serial ELF collection in lung transplant models is crucial.
Purpose of the Study:
- To assess the safety and efficacy of BMS for serial ELF collection in a canine lung transplant model.
- To evaluate the utility of BMS in monitoring molecular changes indicative of lung injury post-transplantation.
- To compare molecular detection in ELF via BMS versus BAL fluid.
Main Methods:
- Serial ELF collection using BMS hourly up to 5 hours post-reperfusion in a canine lung transplant model.
- Measurement of tumor necrosis factor-alpha (TNF-α) levels in ELF using enzyme-linked immunosorbent assay.
- Comparison of TNF-α levels in ELF with those in BAL fluid and a sham-operated group.
Main Results:
- BMS enabled safe, serial ELF collection from grafted lungs without adverse effects.
- Significant elevation of TNF-α was detected in ELF for 5 hours post-reperfusion, correlating with ischemia-reperfusion lung injury.
- TNF-α concentrations in ELF were substantially higher (over 100-fold) than in BAL fluid.
Conclusions:
- BMS is a safe and effective technique for serial ELF collection in lung allografts.
- Temporal monitoring of TNF-α via BMS accurately reflects ischemia-reperfusion lung injury.
- This study pioneers the use of BMS for elucidating pulmonary function dynamics after lung transplantation.
