Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Scientific breakthrough versus successful startup: teaching scientists to navigate bench to business.

Trends in biotechnology·2026

Same author

High-throughput screening of FRET-based proteins using a hyperspectral microcapillary array.

iScience·2026

Same author

IGSF11-VISTA is a critical and targetable immune checkpoint axis in diffuse midline glioma.

Cancer cell·2026

Same author

Dictionary of human intestinal organoid responses to secreted niche factors at single cell resolution.

Nature communications·2026

Same author

Functional NK engagers from OmniClic, a common light-chain platform producing fully human-sequence antibodies in a chicken host species.

mAbs·2025

Same author

A Common PD-Risk <i>GBA1</i> Variant Disrupts LIMP2 Interaction, Impairs Glucocerebrosidase Function, and Drives Lysosomal and Mitochondrial Dysfunction.

bioRxiv : the preprint server for biology·2025

Same journal

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

ACS chemical biology·2026

Same journal

Development of Next-Generation Fluoroacetamidine-Containing Activity-Based Probes for the Selective Labeling of the Protein Arginine Deiminases (PADs).

ACS chemical biology·2026

Same journal

Spectroelectrochemical Insight into Reaction Mechanisms of Cell-Penetrating Peptides on Charged Membrane Surfaces.

ACS chemical biology·2026

Same journal

Metal- and Redox-Dependent Oxytocin Species Differentially Regulate Invasion and Migration in Triple-Negative Breast Cancer.

ACS chemical biology·2026

Same journal

A Systematic Direct-to-Biology Approach Identified Potent Cereblon HaloPROTACs.

ACS chemical biology·2026

Same journal

Resistance-CONKAT-seq Guided Discovery of a ClpP Active Natural Product from a Soil Metagenome.

ACS chemical biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 9, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Engineering High Affinity Protein-Protein Interactions Using a High-Throughput Microcapillary Array Platform.

Sungwon Lim¹, Bob Chen¹, Mihalis S Kariolis¹

¹Department of Bioengineering, ‡Institute for Stem Cell Biology and Regenerative Medicine, §Stanford Photonics Research Center, ∥Chemical Engineering, Stanford University , 450 Serra Mall, Stanford, California 94305, United States.

ACS Chemical Biology

|December 21, 2016

Summary

This summary is machine-generated.

Researchers developed a new protein engineering platform, μSCALE (Microcapillary Single Cell Analysis and Laser Extraction), for rapid affinity maturation. This technology accelerated the engineering of Axl receptor variants to bind Gas6 with 50-fold higher affinity.

More Related Videos

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Related Experiment Videos

Last Updated: Mar 9, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Biochemistry
Molecular Biology
Protein Engineering

Background:

Affinity maturation of protein-protein interactions is crucial for developing therapeutics and research tools.
Traditional methods involve iterative library generation and screening, which can be time-consuming.
High-throughput screening is essential for efficiently identifying improved protein variants.

Purpose of the Study:

To demonstrate the first application of the μSCALE platform for affinity maturation of a protein-protein binding interaction.
To engineer variants of the Axl receptor tyrosine kinase with enhanced binding to its ligand, Gas6.
To validate μSCALE as an efficient tool for directed evolution.

Main Methods:

Development and implementation of the μSCALE (Microcapillary Single Cell Analysis and Laser Extraction) platform.
Generation of protein libraries for iterative rounds of screening.
Engineering of the extracellular domain of Axl receptor tyrosine kinase.
High-throughput screening of protein variants based on binding properties.

Main Results:

Successful application of μSCALE for affinity maturation of the Axl-Gas6 interaction.
Engineered Axl variants exhibited a 50-fold decrease in kinetic dissociation rate (increased binding affinity).
Achieved significant improvement within two weeks through two iterative rounds of library generation and screening.

Conclusions:

The μSCALE platform is a powerful and efficient tool for high-throughput protein engineering and directed evolution.
μSCALE significantly accelerates the process of affinity maturation for protein-protein interactions.
This technology holds promise for the rapid development of proteins with desired binding characteristics.