Related Experiment Video
Updated: Mar 9, 2026

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
Published on: January 3, 2017
Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
Yanlong Yang1, Baoli Yao1, Ming Lei1
1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
Abstract:
Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.
More Related Videos
12:22Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
Published on: August 4, 2018
12:59Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
Published on: February 28, 2021
Related Concept Videos
Confocal Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology